time-dependent changes of autophagy and apoptosis in lipopolysaccharide-induced rat acute lung injury
نویسندگان
چکیده
objective(s): abnormal lung cell death including autophagy and apoptosis is the central feature in acute lung injury (ali). to identify the cellular mechanisms and the chronology by which different types of lung cell death are activated during lipopolysaccharide (lps)-induced ali, we decided to evaluate autophagy (by lc3-ii and autophagosome) and apoptosis (by caspase-3) at different time points after lps treatment in a rat model of lps-induced ali. materials and methods: sprague-dawley rats were randomly divided into two groups: control group and lps group. ali was induced by lps intraperitoneal injection (3 mg/kg). the lung tissues were collected to measure lung injury score by histopathological evaluation, the protein expression of lc3-ii and caspase-3 by western blot, and microstructural changes by electron microscopy analysis. results: during ali, lung cell death exhibited modifications in the death process at different stages of ali. at early stages (1 hr and 2 hr) of ali, the mode of lung cell death started with autophagy in lps group and reached a peak at 2 hr. as ali process progressed, apoptosis was gradually increased in the lung tissues and reached its maximal level at later stages (6 hr), while autophagy was time-dependently decreased. conclusion:these findings suggest that activated autophagy and apoptosis might play distinct roles at different stages of lps-induced ali. this information may enhance the understanding of lung pathophysiology at the cellular level during ali and pulmonary infection, and thus help optimize the timing of innovating therapeutic approaches in future experiments with this model.
منابع مشابه
Time-dependent changes of autophagy and apoptosis in lipopolysaccharide-induced rat acute lung injury
Objective(s): Abnormal lung cell death including autophagy and apoptosis is the central feature in acute lung injury (ALI). To identify the cellular mechanisms and the chronology by which different types of lung cell death are activated during lipopolysaccharide (LPS)-induced ALI, we decided to evaluate autophagy (by LC3-II and autophagosome) and apoptosis (by caspase-3) at different time point...
متن کاملTime-dependent changes of autophagy and apoptosis in lipopolysaccharide-induced rat acute lung injury
OBJECTIVES Abnormal lung cell death including autophagy and apoptosis is the central feature in acute lung injury (ALI). To identify the cellular mechanisms and the chronology by which different types of lung cell death are activated during lipopolysaccharide (LPS)-induced ALI, we decided to evaluate autophagy (by LC3-II and autophagosome) and apoptosis (by caspase-3) at different time points a...
متن کاملTime course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
Objective(s):Therapies with high levels of oxygen are commonly used in the management of critical care. However, prolonged exposure to hyperoxia can cause acute lung injury. Although oxidative stress and inflammation are purported to play an important role in the pathogenesis of acute lung injury, the exact mechanisms are still less known in the hyperoxic acute lung injury (HALI). Materials ...
متن کاملRetracted: Time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
متن کامل
time course changes of oxidative stress and inflammation in hyperoxia-induced acute lung injury in rats
objective(s):therapies with high levels of oxygen are commonly used in the management of critical care. however, prolonged exposure to hyperoxia can cause acute lung injury. although oxidative stress and inflammation are purported to play an important role in the pathogenesis of acute lung injury, the exact mechanisms are still less known in the hyperoxic acute lung injury (hali). materials a...
متن کاملFas/FasL-dependent apoptosis of alveolar cells after lipopolysaccharide-induced lung injury in mice.
To determine the possible contribution of apoptosis in the pathogenesis of acute lung injury (ALI), we investigated Fas antigen (Fas), Fas ligand (FasL), perforin, granzyme A, and granzyme B expressions in a murine model of ALI after intratracheal instillation of Escherichia coli lipopolysaccharide (LPS: 0.3-30 microg) into the left lung. Lung injury, examined by water-to-dry weight ratio and a...
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عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۶، صفحات ۶۳۲-۶۳۷
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